LOCUS       40924_23699       11346 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB710 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11346)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 11346)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 11346)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11346)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..11346
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    338..462
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        499..529
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             583..1239
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             1584..1886
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(1930..2054)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2065..2088
                     /codon_start=1
                     /label=FLAG
                     /note="FLAG(R) epitope tag, followed by an enterokinase
                     cleavage site"
                     /translation="DYKDDDDK"
     terminator      2110..2362
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2396..2412)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    2420..2436
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2444..2474)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2489..2510)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(2546..2798)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(2836..4131)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61187.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(2836..4131)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(4183..4362)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(4868..4892)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5413..6201
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6450..7038
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7224..7364)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(7708..7902)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(7971..9041)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9473..10099)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt cgactctaga gttatcaaca agtttgtaca aaaaagctga
      361 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca
      421 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggcccc tcgaggggcc
      481 gcattaggca ccccaggctt tacactttat gcttccggct cgtataatgt gtggattttg
      541 agttaggatc cgtcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact
      601 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag
      661 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag
      721 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg
      781 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat
      841 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg
      901 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt
      961 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca
     1021 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc
     1081 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg
     1141 ctggcgattc aggttcatca tgccgtttgt gatggcttcc atgtcggcag aatgcttaat
     1201 gaattacaac agtactgcga tgagtggcag ggcggggcgt aaagatctgg atccggctta
     1261 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat
     1321 actgatatgt atacccgaag tatgtcaaaa agaggtatgc tatgaagcag cgtattacag
     1381 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg
     1441 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc
     1501 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc
     1561 tgacgagaac aggggctggt gaaatgcagt ttaaggttta cacctataaa agagagagcc
     1621 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg
     1681 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg
     1741 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg
     1801 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg
     1861 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg
     1921 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta
     1981 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc
     2041 ttgtacaaag tggttgataa cagcgactac aaggatgacg atgacaaggc ttagagctcg
     2101 aatttccccg atcgttcaaa catttggcaa taaagtttct taagattgaa tcctgttgcc
     2161 ggtcttgcga tgattatcat ataatttctg ttgaattacg ttaagcatgt aataattaac
     2221 atgtaatgca tgacgttatt tatgagatgg gtttttatga ttagagtccc gcaattatac
     2281 atttaatacg cgatagaaaa caaaatatag cgcgcaaact aggataaatt atcgcgcgcg
     2341 gtgtcatcta tgttactaga tcgggaattg gttccggaac caattcgtaa tcatggtcat
     2401 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2461 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta ggctgaatta
     2521 ggcgcgccta tttctattaa ttcccgatct agtaacatag atgacaccgc gcgcgataat
     2581 ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg
     2641 actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca
     2701 tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc
     2761 aatcttaaga aactttattg ccaaatgttt gaacgatcgg ggaaattcgc ctcgagtgac
     2821 ttctctgatt gcagactatt tataccaacc agcaagaaag tgtcgaagta tgaagcagaa
     2881 ggcgcaggct agagcctgga aaacgagaag atggatgcaa agagactttt ctgatttagg
     2941 accaaacaac ctcaagtaaa cattgacgag tgtcccatct tttgtgccgg tcagtaatcc
     3001 ggtagcagga tcataccttg ggagacgatg gcgcggacat ttcacaatcc cagcaagctg
     3061 aggaagcgat aatagaaggt tcagaacttg aggaagaaag aagatgagga gggtttcact
     3121 gaagtgaccc agaataccaa caactgccat agtcattccg gcgaagactg tgtaagtgtc
     3181 tccaacaaaa actgaagaag gataccaatt gtaagcaagc attgccaagg atgtggccat
     3241 cagcggttga gtaagaaata tcgagaaggc atgagcttgg tgatactcgg gatcaacaga
     3301 tgctccgatt tgcataacgt tgtgtatcag gatagcagcg gcaataacaa ctgtttgacc
     3361 gatttcaagg ccattcagac cagcgtggat gttaatagag tttgtacaaa agacagcaag
     3421 tagccccata tataacttat atatccttcc tagattaagc acttccaagc caatataagc
     3481 aacaagaggt tttggtataa caatagttgt atgaccagca taagccatca aaagtggaag
     3541 ggtagcaaaa gatggcagga caagttttac tctccaaggc acatcaagga catcatctac
     3601 aaatccaagc aaaatcatga agcaaataga tgctagtgct gcattatact ccacaagcca
     3661 attagaatct tcagtgaagt taaaatactg aaatattatc gccacgatca aaaagacaat
     3721 accaacaaca atacccaatg actctggcac tttaatatct ccttgaggcg tgcctctttt
     3781 gttgatatca aaaccaaaca tgttacgtct cagaacgtat ctggcagtaa caggaatcat
     3841 tttgagagtg acgaaaaacc caaccaagct aagccccgcg ttaattagaa tcgatcgctt
     3901 gagttcatta tcaaccttgt agtgaaaaca gagaaggtat agatagagcg aacagagaag
     3961 agtagagatc acgaagatca ctcctaattt cggcggcgcg agacgaaatt cctcaccgga
     4021 agcagaaacc gtctttcggg tcatcttctg ctccgatggc gctgagttgg gttccgacgg
     4081 gtccggtttg ttgggaattg agatcgatga agctctctta cgagctgcca tatcttattg
     4141 ccccccggga tcagatctgg attgagagtg aatatgagac tctaattgga taccgagggg
     4201 aatttatgga acgtcagtgg agcatttttg acaagaaata tttgctagct gatagtgacc
     4261 ttaggcgact tttgaacgcg caataatggt ttctgacgta tgtgcttagc tcattaaact
     4321 ccagaaaccc gcggctgagt ggctccttca acgttgcggt tctgtcagtt ccaaacgtaa
     4381 aacggcttgt cccgcgtcat cggcgggggt cataacgtga ctcccttaat tctccgctca
     4441 tgatcttgat cccctgcgcc atcagatcct tggcggcaag aaagccatcc agtttacttt
     4501 gcagggcttc ccaaccttac cagagggcgc cccagctggc aattccggtt cgcttgctgt
     4561 ccataaaacc gcccagtcta gctatcgcca tgtaagccca ctgcaagcta cctgctttct
     4621 ctttgcgctt gcgttttccc ttgtccagat agcccagtag ctgacattca tccggggtca
     4681 gcaccgtttc tgcggactgg ctttctacgt gttccgcttc ctttagcagc ccttgcgccc
     4741 tgagtgcttg cggcagcgtg aagctgatcc gtcgagatta atagaaataa attcagccta
     4801 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     4861 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     4921 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     4981 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     5041 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     5101 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     5161 tgttcggttc gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc
     5221 ttgaccgaac gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt
     5281 ttttttgggg tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt
     5341 cgatgtttga tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca
     5401 aagttaaaca tcatggggga agcggtgatc gccgaagtat cgactcaact atcagaggta
     5461 gttggcgtca tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc
     5521 gcagtggatg gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta
     5581 aggcttgatg aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc
     5641 cctggagaga gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc
     5701 attccgtggc gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac
     5761 attcttgcag gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca
     5821 aaagcaagag aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg
     5881 gttcctgaac aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg
     5941 cccgactggg ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc
     6001 gcagtaaccg gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg
     6061 ccggcccagt atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa
     6121 gatcgcttgg cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag
     6181 atcaccaagg tagtcggcaa ataatgtcta gctagaaatt cgttcaagcc gacgccgctt
     6241 cgcggcgcgg cttaactcaa gcgttagatg cactaagcac ataattgctc acagccaaac
     6301 tatcaggtca agtctgcttt tattattttt aagcgtgcat aataagccct acacaaattg
     6361 ggagatatat catgcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     6421 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     6481 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     6541 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     6601 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     6661 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     6721 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     6781 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     6841 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     6901 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     6961 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     7021 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     7081 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     7141 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     7201 gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt
     7261 atttcacacc gcatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc
     7321 cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa
     7381 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     7441 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     7501 ggcagggtgc cttgatgtgg gcgccggcgg tcgagtggcg acggcgcggc ttgtccgcgc
     7561 cctggtagat tgcctggccg taggccagcc atttttgagc ggccagcggc cgcgataggc
     7621 cgacgcgaag cggcggggcg tagggagcgc agcgaccgaa gggtaggcgc tttttgcagc
     7681 tcttcggctg tgcgctggcc agacagttat gcacaggcca ggcgggtttt aagagtttta
     7741 ataagtttta aagagtttta ggcggaaaaa tcgccttttt tctcttttat atcagtcact
     7801 tacatgtgtg accggttccc aatgtacggc tttgggttcc caatgtacgg gttccggttc
     7861 ccaatgtacg gctttgggtt cccaatgtac gtgctatcca caggaaagag accttttcga
     7921 cctttttccc ctgctagggc aatttgccct agcatctgct ccgtacatta ggaaccggcg
     7981 gatgcttcgc cctcgatcag gttgcggtag cgcatgacta ggatcgggcc agcctgcccc
     8041 gcctcctcct tcaaatcgta ctccggcagg tcatttgacc cgatcagctt gcgcacggtg
     8101 aaacagaact tcttgaactc tccggcgctg ccactgcgtt cgtagatcgt cttgaacaac
     8161 catctggctt ctgccttgcc tgcggcgcgg cgtgccaggc ggtagagaaa acggccgatg
     8221 ccgggatcga tcaaaaagta atcggggtga accgtcagca cgtccgggtt cttgccttct
     8281 gtgatctcgc ggtacatcca atcagctagc tcgatctcga tgtactccgg ccgcccggtt
     8341 tcgctcttta cgatcttgta gcggctaatc aaggcttcac cctcggatac cgtcaccagg
     8401 cggccgttct tggccttctt cgtacgctgc atggcaacgt gcgtggtgtt taaccgaatg
     8461 caggtttcta ccaggtcgtc tttctgcttt ccgccatcgg ctcgccggca gaacttgagt
     8521 acgtccgcaa cgtgtggacg gaacacgcgg ccgggcttgt ctcccttccc ttcccggtat
     8581 cggttcatgg attcggttag atgggaaacc gccatcagta ccaggtcgta atcccacaca
     8641 ctggccatgc cggccggccc tgcggaaacc tctacgtgcc cgtctggaag ctcgtagcgg
     8701 atcacctcgc cagctcgtcg gtcacgcttc gacagacgga aaacggccac gtccatgatg
     8761 ctgcgactat cgcgggtgcc cacgtcatag agcatcggaa cgaaaaaatc tggttgctcg
     8821 tcgcccttgg gcggcttcct aatcgacggc gcaccggctg ccggcggttg ccgggattct
     8881 ttgcggattc gatcagcggc cgcttgccac gattcaccgg ggcgtgcttc tgcctcgatg
     8941 cgttgccgct gggcggcctg cgcggccttc aacttctcca ccaggtcatc acccagcgcc
     9001 gcgccgattt gtaccgggcc ggatggtttg cgaccgctca cgccgattcc tcgggcttgg
     9061 gggttccagt gccattgcag ggccggcaga caacccagcc gcttacgcct ggccaaccgc
     9121 ccgttcctcc acacatgggg cattccacgg cgtcggtgcc tggttgttct tgattttcca
     9181 tgccgcctcc tttagccgct aaaattcatc tactcattta ttcatttgct catttactct
     9241 ggtagctgcg cgatgtattc agatagcagc tcggtaatgg tcttgccttg gcgtaccgcg
     9301 tacatcttca gcttggtgtg atcctccgcc ggcaactgaa agttgacccg cttcatggct
     9361 ggcgtgtctg ccaggctggc caacgttgca gccttgctgc tgcgtgcgct cggacggccg
     9421 gcacttagcg tgtttgtgct tttgctcatt ttctctttac ctcattaact caaatgagtt
     9481 ttgatttaat ttcagcggcc agcgcctgga cctcgcgggc agcgtcgccc tcgggttctg
     9541 attcaagaac ggttgtgccg gcggcggcag tgcctgggta gctcacgcgc tgcgtgatac
     9601 gggactcaag aatgggcagc tcgtacccgg ccagcgcctc ggcaacctca ccgccgatgc
     9661 gcgtgccttt gatcgcccgc gacacgacaa aggccgcttg tagccttcca tccgtgacct
     9721 caatgcgctg cttaaccagc tccaccaggt cggcggtggc ccatatgtcg taagggcttg
     9781 gctgcaccgg aatcagcacg aagtcggctg ccttgatcgc ggacacagcc aagtccgccg
     9841 cctggggcgc tccgtcgatc actacgaagt cgcgccggcc gatggccttc acgtcgcggt
     9901 caatcgtcgg gcggtcgatg ccgacaacgg ttagcggttg atcttcccgc acggccgccc
     9961 aatcgcgggc actgccctgg ggatcggaat cgactaacag aacatcggcc ccggcgagtt
    10021 gcagggcgcg ggctagatgg gttgcgatgg tcgtcttgcc tgacccgcct ttctggttaa
    10081 gtacagcgat aaccttcatg cgttcccctt gcgtatttgt ttatttactc atcgcatcat
    10141 atacgcagcg accgcatgac gcaagctgtt ttactcaaat acacatcacc tttttagacg
    10201 gcggcgctcg gtttcttcag cggccaagct ggccggccag gccgccagct tggcatcaga
    10261 caaaccggcc aggatttcat gcagccgcac ggttgagacg tgcgcgggcg gctcgaacac
    10321 gtacccggcc gcgatcatct ccgcctcgat ctcttcggta atgaaaaacg gttcgtcctg
    10381 gccgtcctgg tgcggtttca tgcttgttcc tcttggcgtt cattctcggc ggccgccagg
    10441 gcgtcggcct cggtcaatgc gtcctcacgg aaggcaccgc gccgcctggc ctcggtgggc
    10501 gtcacttcct cgctgcgctc aagtgcgcgg tacagggtcg agcgatgcac gccaagcagt
    10561 gcagccgcct ctttcacggt gcggccttcc tggtcgatca gctcgcgggc gtgcgcgatc
    10621 tgtgccgggg tgagggtagg gcgggggcca aacttcacgc ctcgggcctt ggcggcctcg
    10681 cgcccgctcc gggtgcggtc gatgattagg gaacgctcga actcggcaat gccggcgaac
    10741 acggtcaaca ccatgcggcc ggccggcgtg gtggtgtcgg cccacggctc tgccaggcta
    10801 cgcaggcccg cgccggcctc ctggatgcgc tcggcaatgt ccagtaggtc gcgggtgctg
    10861 cgggccaggc ggtctagcct ggtcactgtc acaacgtcgc cagggcgtag gtggtcaagc
    10921 atcctggcca gctccgggcg gtcgcgcctg gtgccggtga tcttctcgga aaacagcttg
    10981 gtgcagccgg ccgcgtgcag ttcggcccgt tggttggtca agtcctggtc gtcggtgctg
    11041 acgcgggcat agcccagcag gccagcggcg gcgctcttgt tcatggcgta atgtctccgg
    11101 ttctagtcgc aagtattcta ctttatgcga ctaaaacacg cgacaagaaa acgccaggaa
    11161 aagggcaggg cggcagcctg tcgcgtaact taggacttgt gcgacatgtc gttttcagaa
    11221 gacggctgca ctgaacgtca gaagccgact gcactatagc agcggagggg ttggatcaaa
    11281 gtactttgat cccgagggga accctgtggt tggcatgcac atacaaatgg acgaacggat
    11341 aaacct
//
